烟草<i>CONSTANS-like</i>基因家族的鉴定与分析

烟草CONSTANS-like基因家族的鉴定与分析

张雅文¹^,², 梁山¹, 徐国云³, 郭无瑕²^,⁴^,^*(

), 邓书林²^,⁴^,^*(

)

Genome-wide Identification and Analysis of CONSTANS-like Gene Family in Nicotiana tabacum

Yawen Zhang¹^,², Shan Liang¹, Guoyun Xu³, Wuxia Guo²^,⁴^,^*(

), Shulin Deng²^,⁴^,^*(

)

图4. 烟草所有COL基因(A)和5对部分同源基因(B)-(F)在低温处理下不同发育阶段叶片中的表达
FC(S): 12°C处理下6-7叶期的表达差异倍数; FC(F): 12°C处理下现蕾期的表达差异倍数; ck_S: 26°C下6-7叶期烟草; ck_F: 26°C下现蕾期烟草; cold_S: 12°C处理后6-7叶期烟草; cold_F: 12°C处理后现蕾期烟草; 黑色: 来自父本的基因; 灰色: 来自母本的基因。相对表达水平为3次生物学重复的平均值, 误差线代表标准差, 采用Student’s t-test对部分同源基因对进行差异显著性检验, * P<0.05, ** P<0.01, *** P<0.001, **** P<0.0001, ns表示无显著性差异。

Figure 4. Expression of all COL genes (A) and five homologous genes pairs (B)-(F) in tobacco leaves at different developmental stages under cold treatment
FC(S): Fold change at 6-7 leaf stage under 12°C treatment; FC(F): Fold change at budding stage under 12°C treatment; ck_S: 6-7 leaf stage under 26°C; ck_F: Budding stage under 26°C; cold_S: 6-7 leaf stage under 12°C treatment; cold_F: Budding stage under 12°C treatment; Black: Orthologs from paternal plant; Gray: Orthologs from maternal plant; the relative expression level is the average of 3 biological replicates, the error bar represents standard deviation, and Student’s t-test is used for significance test, * P<0.05, ** P<0.01, *** P<0.001, **** P<0.0001, and ns means not significant.