水稻籽粒镉积累QTL定位及候选基因分析

潘晨阳, 叶涵斐, 周维永, 王盛, 李梦佳, 路梅, 李三峰, 朱旭东, 王跃星, 饶玉春, 戴高兴

QTL Mapping of Candidate Genes Involved in Cd Accumulation in Rice Grain

Chenyang Pan, Hanfei Ye, Weiyong Zhou, Sheng Wang, Mengjia Li, Mei Lu, Sanfeng Li, Xudong Zhu, Yuexing Wang, Yuchun Rao, Gaoxing Dai

表1 qRT-PCR所用引物序列

Table 1 Primer sequences for qRT-PCR

Primer name Sequence (5'-3') Tm (°C) Length (bp) LOC_Os02g50240-F-qrt CTTCAAGGACCCATTCAGGA 60.04 113 LOC_Os02g50240-R-qrt TGAAGATCTTGGCAGCATTG 59.95 LOC_Os02g52780-F-qrt GTTTTCAAAGGTGGGCTGAG 59.71 148 LOC_Os02g52780-R-qrt CCTCAGCTTCCAATTCCATC 59.68 LOC_Os09g31200-F-qrt GCGACAAGAAGGATCAGGAG 59.95 117 LOC_Os09g31200-R-qrt AGAAGCAGTTCTGGCAGAGG 59.75 LOC_Os09g35030-F-qrt GGATCAAGCAGGAGATGAGC 59.92 135 LOC_Os09g35030-R-qrt GCCTCGTCTCCCTGAACTT 59.39 LOC_Os09g37949-F-qrt GGAAATCTGCCGATCAGTGT 60.08 123 LOC_Os09g37949-R-qrt GGGTCCTTCGATGGGTTTAT 60.02