香鳞毛蕨的组织培养和快速繁殖体系构建

张冬瑞, 卜志刚, 陈玲玲, 常缨

Establishment of a Tissue Culture and Rapid Propagation System of Dryopteris fragrans

Dongrui Zhang, Zhigang Bu, Lingling Chen, Ying Chang

表3 不同激素浓度对香鳞毛蕨茎尖生长点愈伤组织诱导的影响

Table 3 Callus induction of Dryopteris fragrans with various concentrations of hormones from the shoot tip apex

Treatments 6-BA (mg·L-1) 2,4-D (mg·L-1) Induction period (d) Induction rate (%) Browning rate (%) Callus growth 1 0 0 30 0 0 2 3.0 0.5 30 43.33±3.33 38.61±2.98 ++ 3 3.0 1.0 30 33.33±3.33 36.94±7.24 ++ 4 3.0 1.5 30 17.78±1.92 68.89±10.18 + 5 2.0 0.5 30 96.67±5.77 3.33±5.77 ++++ 6 2.0 1.0 30 81.11±1.92 28.67±6.35 +++ 7 2.0 1.5 30 76.67±5.77 48.21±9.94 ++ 8 1.5 0.5 30 83.33±5.77 31.94±6.36 +++ 9 1.5 1.0 30 80.00±0.00 45.83±7.22 ++ 10 1.5 1.5 30 76.67±5.77 52.38±4.12 ++ 11 1.0 0.5 30 86.67±5.77 54.17±9.11 ++ 12 1.0 1.0 30 86.67±5.77 57.87±4.01 ++ 13 1.0 1.5 30 73.33±5.77 58.92±3.09 ++