万寿菊再生体系的建立及优化
王亚琴1,2, 韦陆丹1, 王文静1, 刘宝骏1, 张春玲1, 张俊卫1, 何燕红1,*()
The Establishment and Optimization of a Regeneration System for Marigold (Tagetes erecta)
Yaqin Wang1,2, Ludan Wei1, Wenjing Wang1, Baojun Liu1, Chunling Zhang1, Junwei Zhang1, Yanhong He1,*()

图1. 万寿菊的叶片再生过程
(A) 易再生基因型: 里程碑·黄色; (B) 中等再生基因型: 自豪·橙黄改良; (C) 难再生基因型: 奇迹·黄色; (D) 不能再生基因型: 安提瓜·桔色; (E) 生长在MS+3 mg·L?1 6-BA+3 mg·L?1 IAA+3 g·L?1植物凝胶+30 g·L?1蔗糖培养基的里程碑·黄色叶片; (F) 生长在MS+0.3 mg·L?1 ZT+2.0 mg·L?1 IAA+3 g·L?1植物凝胶+30 g·L?1蔗糖培养基的里程碑·黄色叶片; (G) 生长在MS+0.6 mg·L?1 ZT+0.5 mg·L?1 IAA+3 g·L?1植物凝胶+30 g·L?1蔗糖培养基的里程碑·黄色叶片; (H) 生长在MS+0.2 mg·L?1 TDZ+0.5 mg·L?1 IBA+3 g·L?1植物凝胶+30 g·L?1蔗糖培养基的里程碑·黄色叶片; (I) 生长在MS+0.2 mg·L?1 TDZ+0.5 mg·L?1 IBA+8 g·L?1琼脂+30 g·L?1蔗糖培养基的里程碑·黄色叶片; (J) 生长在MS+0.2 mg·L?1 TDZ+0.5 mg·L?1 IBA+8 g·L?1琼脂+40 g·L?1蔗糖培养基的里程碑·黄色叶片; (K) 在MS1培养基上生根伸长的再生苗; (L) 移栽成活的再生植株。(A)-(K) Bars=1 cm; (L) Bar=2 cm

Figure 1. Leaves regenerations process of marigold
(A) An easy regeneration genotype: Milestone·Yellow; (B) A moderate regeneration genotype: Proud·Orange-yellow; (C) A difficult regeneration genotype: Miracle·Yellow; (D) An unregenerable genotype: Antigua·Orange; (E) The leaves of Milestone·Yellow on the MS medium supplied with 3 mg·L?1 6-BA, 3 mg·L?1 IAA, 3 g·L?1 phytagel, and 30 g·L?1 sucrose; (F) The leaves of Milestone·Yellow on the MS medium supplied with 0.3 mg·L?1 ZT, 2.0 mg·L?1 IAA, 3 g·L?1 phytagel, and 30 g·L?1 sucrose; (G) The leaves of Milestone·Yellow on the MS medium supplied with 0.6 mg·L?1 ZT, 0.5 mg·L?1 IAA, 3 g·L?1 phytagel, and 30 g·L?1 sucrose; (H) The leaves of Milestone·Yellow on the MS medium supplied with 0.2 mg·L?1 TDZ, 0.5 mg·L?1 IBA, 3 g·L?1 phytagel, and 30 g·L?1 sucrose; (I) The leaves of Milestone·Yellow on the MS medium supplied with 0.2 mg·L?1 TDZ, 0.5 mg·L?1 IBA, 8 g·L?1 agar, and 30 g·L?1 sucrose; (J) The leaves of Milestone·Yellow on the MS medium supplied with 0.2 mg·L?1 TDZ, 0.5 mg·L?1 IBA, 8 g·L?1 agar, and 40 g·L?1 sucrose; (K) Regenerated shoot extension and rooting culture on MS1 medium; (L) A living regenerating transplanted plants. (A)-(K) Bars=1 cm; (L) Bar=2 cm