干旱胁迫下表观遗传机制对转C4型PEPC基因水稻种子萌发的影响

宋凝曦, 谢寅峰, 李霞

Effects of Epigenetic Mechanisms on C4 Phosphoenolpyruvate Carboxylase Transgenic Rice (Oryza sativa) Seed Germination Under Drought Stress

Ningxi Song, Yingfeng Xie, Xia Li

表1 引物序列

Table 1 Primers used in this study

Gene Forward primer (5'-3') Reverse primer (5'-3') Actin CCCTCAAACATCGGTATGGA TTGATCTTCATGCTGCTTGG OsK1a AACCAGAGGTAACAGGCAGG AACCAGAGGTAACAGGCAGG OsK24 CGTGTTGGCTTCAGTGAAT CCTTCTCTATCTAAGGGCCG OsK35 TTGTGTTGGCTTCAGTGAAA CCTTCGCTGTCTAAGGACTG C4-PEPC CCCACTATCCTTCGCAAGAC CTAGCCAGTGTTCTGCATGCCGG Osppc2a CTGGTTGAGATGGTTTTCGC GGTGTGAATTCAGGCACTTC SAPK8 ATAGATGATAATGTCCAGCG GTTCCTACAGTGGATTTTGG SAPK9 CACAGCAACGCCGTCTCC CACACTTCCACCGCTACCAA SAPK10 TGCTGATGTGTGGTCGTGTG TGCTGGTATGGTCGCCTCT SR40 CAATCTGGGGACTGCTTTC TCCTGCTTGGGCTTTTACT SR33 ATATTGCCTGCTACCCGAAAG CAGAGCAGCACCCAGTTTATTAC OsAmy1A TTTCGGTCCTCATCGTCCTCC TCCACGACTCCCAGTTGAATC OsAmy1C TGGTATCGATCAGAAACCGGC GTCCGACCTTCGTGATGACC OsAmy3C AAGCATTCCACCACAATGAGC AGGAAGTTGTACCACCCACC OsAmy3E TCACCCTGTGTTGTGTCGTT AAAGTTGTACCACCCGCCTT