长白落叶松体胚发生再生体系优化

刘建飞, 刘炎, 刘克俭, 池阳, 霍志发, 霍永洪, 由香玲

Optimization of the Regeneration System from Somatic Embryogenesis in Larix olgensis

Jianfei Liu, Yan Liu, Kejian Liu, Yang Chi, Zhifa Huo, Yonghong Huo, Xiangling You

表2 不同浓度配比的植物生长调节剂对长白落叶松体胚发生率和体胚数量的影响(平均值±标准差)

Table 2 Effects of plant growth regulators at different concentration ratios on somatic embryogenesis and somatic embryo amount in Larix olgensis (means±SD)

The combination of plant growth regulators in the medium Somatic embryogenesis (%) Somatic embryo amount (numbers·g-1) 0.1 mg?L-1 2,4-D+0.04 mg·L-1 BA+0.02 mg·L-1 KT 45.27±23.31 16.47±1.67 0.5 mg?L-1 NAA+0.5 mg·L-1 BA+0.5 mg·L-1 KT 52.65±31.83 b 25.31±9.85 c 0.4 mg?L-1 NAA+0.5 mg·L-1 BA+0.5 mg·L-1 KT 57.38±15.41 ab 31.53±5.37 b 0.3 mg?L-1 NAA+0.5 mg·L-1 BA+0.5 mg·L-1 KT 64.41±11.79 a 39.74±4.29 ab 0.2 mg?L-1 NAA+0.5 mg·L-1 BA+0.5 mg·L-1 KT 72.36±10.57 a 48.56±3.77 a 0.1 mg?L-1 NAA+0.5 mg·L-1 BA+0.5 mg·L-1 KT 63.70±43.86 a 41.92±7.22 a