根尖整体透明技术改良
马龙, 李桂林, 李师鹏, 蒋苏*(
)
)
An Improved Protocol for Whole Mount Clearing of Plant Root Tip
Long Ma, Guilin Li, Shipeng Li, Su Jiang*()
)
图5. 透明液pH值对拟南芥根尖透明的影响
分别采用透明液HCG-1 (A)和HCG-2 (B)在pH1.2/1.8、pH5.8和pH7.2条件下, 对拟南芥幼苗透明5分钟后, 根尖伸长区表皮细胞质壁分离比例和静止中心(QC)细胞清晰的根尖所占比例。不同大写字母表示差异极显著(P<0.01)。Bars=50 μm
Figure 5. The effects of pH values on Arabidopsis thaliana root tip clearing
Proportion of plasmolytic cells and proportion of root tips with clear quiescent center (QC) cells of Arabidopsis thaliana seedlings cleared by HCG-1 (A) and HCG-2 (B) for 5 min under pH1.2/1.8, pH5.8, pH7.2, respectively. Different uppercase letters indicate extremely significant differences (P<0.01). Bars=50 μm
