根尖整体透明技术改良

马龙, 李桂林, 李师鹏, 蒋苏

An Improved Protocol for Whole Mount Clearing of Plant Root Tip

Long Ma, Guilin Li, Shipeng Li, Su Jiang

表1 原始pH、拟南芥培养基常用pH、中性pH透明液HCG-1和HCG-2

Table 1 Solution HCG-1 and HCG-2 in original pH, the pH used for Arabidopsis thaliana culture and neutral pH

No. HCG-1 Composition pH 1 H2O 9 mL 1.2 Chloral hydrate 24 g Glycerol 3 mL 2 H2O 9 mL 5.8 Chloral hydrate 24 g Glycerol 3 mL 3 H2O 9 mL 7.2 Chloral hydrate 24 g Glycerol 3 mL HCG-2 Composition pH 4 H2O 9 mL 1.8 Chloral hydrate 12 g Glycerol 3 mL 5 H2O 9 mL 5.8 Chloral hydrate 12 g Glycerol 3 mL 6 H2O 9 mL 7.2 Chloral hydrate 12 g Glycerol 3 mL