早花百子莲叶片器官发生和胚胎发生再生体系的建立
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岳建华 1,3, 董艳 2, 王小画 1, 孙佩霞 1, 王思颖 1, 张新年 1, 张琰 1,3,*(  )
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A Regeneration System for Organogenesis and Somatic Embryogenesis Using Leaves of Agapanthus praecox as Explants
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Jianhua Yue 1,3, Yan Dong 2, Xiaohua Wang 1, Peixia Sun 1, Siying Wang 1, Xinnian Zhang 1, Yan Zhang 1,3,*(  )
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图1. 早花百子莲以叶片为外植体诱导植株再生 (A) 叶片外植体; (B) 愈伤组织; (C) 不定芽诱导; (D) 胚性愈伤组织诱导; (E) 不定芽见光后转绿; (F) 球形胚; (G) 器官途径植株再生; (H) 胚胎途径经棒状胚阶段的植株再生; (I) 器官苗增殖; (J) 器官苗根系; (K) 体胚苗增殖; (L) 体胚苗根系; (M) 再生幼苗移栽与驯化; (N) 移栽苗根系。(A)-(D) Bars=0.5 cm; (E)-(L) Bars=1 cm; (M) Bar=5 cm; (N) Bar=10 cm
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Figure 1. Plant regeneration of Agapanthus praecox via leaf explants (A) Leaf explants; (B) Callus; (C) Adventitious bud induction; (D) Embryogenic callus induction; (E) Adventitious bud turns green when exposure to light; (F) Globular embryos; (G) Plant regeneration via organogenesis; (H) Plant regeneration via club-shaped embryo in somatic embryogenesis; (I) Proliferation of plantlets via organogenesis; (J) The root system of plantlets via organogenesis; (K) Proliferation of plantlets via somatic embryogenesis; (L) The root system of plantlets via somatic embryogenesis; (M) Transplanting and acclimatization of regenerated plantlets; (N) The root system of transplanted plantlets. (A)-(D) Bars= 0.5 cm; (E)-(L) Bars=1 cm; (M) Bar=5 cm; (N) Bar=10 cm
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