水稻稻瘟病和纹枯病抗性鉴定方法
贺闽1, 尹俊杰1, 冯志明2, 朱孝波1, 赵剑华2, 左示敏2,*(
), 陈学伟1,*()
), 陈学伟1,*()
Methods for Evaluation of Rice Resistance to Blast and Sheath Blight Diseases
Min He1, Junjie Yin1, Zhiming Feng2, Xiaobo Zhu1, Jianhua Zhao2, Shimin Zuo2,*(), Xuewei Chen1,*()
), Xuewei Chen1,*()
图4. 水稻离体叶片的点滴接种及稻瘟病病斑
(A) 采用10 μL移液器枪头对标1.5 cm的刻度戳伤水稻叶片; (B) 戳伤之后漂浮于平皿6-BA溶液中的水稻叶片; (C) 对戳伤叶片进行点滴接种; (D) 点滴接种之后的水稻叶片; (E) 水稻叶片上的病斑(Bar=1 cm)。
Figure 4. Evaluation of blast disease resistance by inoculation of detached rice leaves
(A) Punching detached rice leaves using 10 μL pipette tips with 1.5 cm scale; (B) Rice leaves put on the surface of 6-BA solution after punching; (C) Inoculation of punched leaves by spotting spore suspension; (D) Rice leaves inoculated with spore suspension of Magnaporthe oryzae; (E) Blast lesions formed on detached rice leaves (Bar=1 cm).
