水稻稻瘟病和纹枯病抗性鉴定方法
贺闽1, 尹俊杰1, 冯志明2, 朱孝波1, 赵剑华2, 左示敏2,*(
), 陈学伟1,*()
), 陈学伟1,*()
Methods for Evaluation of Rice Resistance to Blast and Sheath Blight Diseases
Min He1, Junjie Yin1, Zhiming Feng2, Xiaobo Zhu1, Jianhua Zhao2, Shimin Zuo2,*(), Xuewei Chen1,*()
), Xuewei Chen1,*()
图1. 水稻幼苗期接种稻瘟病菌进行抗性鉴定及典型病斑
(A) 苗床上分小区播种水稻种子; (B) 生长2周的幼苗期水稻; (C) CM平板上的稻瘟病菌菌落; (D) 番茄燕麦产孢平板上生长的稻瘟病菌; (E) 对水稻小区喷雾接种孢悬液; (F) 接种6天后稻瘟病的发病情况; (G) 水稻叶片上产生的典型纺锤形病斑(Bar=1 cm)。
Figure 1. Spraying inoculation at rice seedling stage for evaluation of blast disease resistance and typical rice blast symptom
(A) Sowing rice seeds on seedbeds; (B) Rice seedlings grown for two weeks; (C) Magnaporthe oryzae colony grown on CM agar medium; (D) M. oryzae sporulation on oat tomato agar medium; (E) Inoculation of rice seedlings by spraying spore suspension; (F) Development of blast lesions six-day post inoculation; (G) Typical blast lesions formed on rice leaves (Bar=1 cm).
