水稻稻瘟病和纹枯病抗性鉴定方法

贺闽, 尹俊杰, 冯志明, 朱孝波, 赵剑华, 左示敏, 陈学伟

Methods for Evaluation of Rice Resistance to Blast and Sheath Blight Diseases

Min He, Junjie Yin, Zhiming Feng, Xiaobo Zhu, Jianhua Zhao, Shimin Zuo, Xuewei Chen

表1 稻瘟病菌和纹枯病菌生长培养所需相关试剂配方

Table 1 Reagent formulation used for culturing Magnaporthe oryzae and Rhizoctonia solani

Solution Composition Amount 20× Nitrate salts (1 L) NaNO3 120 g KCl 10.4 g MgSO4.7H2O 10.4 g KH2PO4 30.4 g Vitamin solution (1 L) Biotin 0.1 g Pyridoxin 0.1 g Thiamine 0.1 g Riboflavin 0.1 g p-aminobenzoic acid 0.1 g Nicotinic acid 0.1 g Trace elements (100 mL) ZnSO4.7H2O 2.2 g H3BO3 1.1 g MnCl2.4H2O 0.5 g FeSO4.7H2O 0.5 g CoCl2.6H2O 0.17 g CuSO4.5H2O 0.16 g Na2MoO4.2H2O 0.15 g CM agar medium (1 L, pH6.5) Glucose 10 g Peptone 2 g Yeast extract 1 g Casamino acids 1 g 20× Nitrate salts 50 mL Vitamin solution 1 mL Trace elements 1 mL Agar 15 g Oat tomato agar (1 L) Oatmeal 40 g, collect the liquid filter after boiling Fresh tomato juice 150 mL Agar 15 g PDA medium (1 L) Potato dextrose broth 24 g Agar 20 g