图3. 盐胁迫下AtR8 lncRNA缺失抑制拟南芥种子萌发
(A) Northern分析鉴定AtR8 lncRNA缺失突变体atr8 (以5.8S rRNA作为上样对照); (B) 不同浓度NaCl处理下, 野生型和atr8种子生长状况及萌发率统计分析(数据为3次独立试验的平均值, 误差为标准误); (C) 不同浓度NaCl处理下, 野生型和atr8萌发7天种子的鲜重及干重(数值为3次独立试验的平均值, 误差为标准误, 星号表示atr8与野生型的显著性差异(t-检验, *P<0.05, **P<0.01))。WT: 野生型

Figure 3. Loss of AtR8 lncRNA inhibits Arabidopsis thaliana seed germination under salt stress
(A) Northern blotting analysis of the AtR8 lncRNA loss-of-function mutant atr8 (5.8S rRNA was used as the loading control); (B) Statistical analysis of growth and germination rate of the wild-type and atr8 seeds under different concentrations of NaCl (data are average of three independent experiments, and bars indicate standard error); (C) Fresh and dry weight of the wild-type and atr8 seeds after 7 d of germination under NaCl treatment (the values are average of three independent experiments, and bars indicate standard error (the asterisk indicate significant differences between atr8 and wild type (t-test, * P < 0.05, ** P < 0.01)). WT: Wild type