图2. 拟南芥种子萌发过程中AtR8 lncRNA表达响应NaCl逆境胁迫
(A) AtR8 lncRNA与UCC盐胁迫响应元件的序列比较分析(USE、TATA启动子序列为大写、加粗并加框, AtR8 lncRNA转录区域为大写并加粗, 保守的盐胁迫响应元件用星号标注)。(B) AtR8 lncRNA二级结构中盐胁迫响应元件存在位置的RNAlogo预测(盐胁迫响应元件为大写、加粗并用箭头指出)。(C) 拟南芥种子萌发过程中不同浓度NaCl处理下AtR8 lncRNA表达特性的Northern分析, 以5.8S rRNA作为上样对照。下方为Northern半定量分析, 2个独立的实验给出了相似的结果, 并显示了1个代表性的例子。值为平均值±标准误(t-检验, *P<0.05, **P<0.01)。(D) 拟南芥种子萌发过程中150 mmol·L^-1 NaCl处理不同时间AtR8 lncRNA表达特性的Northern分析, 以5.8S rRNA作为上样对照。下方为Northern半定量分析, 2个独立的实验给出了相似的结果, 并显示了1个代表性的例子。值为平均值±标准误(t-检验, **P<0.01)。

Figure 2. AtR8 lncRNA expression during seed germination of Arabidopsis thaliana after NaCl treatment
(A) Sequence comparison between AtR8 lncRNA and UCC salt stress-responsive element (the USE and TATA promoter sequences are capitalized, bloded and framed; the AtR8 lncRNA transcriptional region is capitalized and bolded; and the conserved salt stress-responsive element is marked with asterisk). (B) The location of the salt stress-responsive element in the secondary structure of AtR8 lncRNA predicted by RNAlogo (the salt stress-responsive motif is capitalized, bloded and indicated with an arrow). (C) Northern blotting analysis of AtR8 lncRNA expression in germinating seeds under different NaCl treatments, 5.8S rRNA was used as a loading control. The lower panel shows semi-quantitative analysis of the Northern blotting signals. Two independent experiments gave similar results, and a representative example is shown. Values are means ± SE (t-test, *P<0.05, **P<0.01). (D) Northern blotting analysis of AtR8 lncRNA in germinating seeds under different periods of 150 mmol·L^-1 NaCl treatment, 5.8S rRNA was used as a loading control. The lower panel shows semi-quantitative analysis of the Northern blotting signals. Two independent experiments gave similar results, and a representative example is shown. Values are means ± SE (t-test, **P<0.01).