毛竹不同截短U3启动子的克隆及表达分析

凡惠金,金康鸣,卓仁英,乔桂荣

Cloning and Expression Analysis of Different Truncated U3 Promoters in Phyllostachys edulis

Huijin Fan,Kangming Jin,Renying Zhuo,Guirong Qiao

表1 本研究中所使用的引物序列

Table 1 Primer sequences used in this study

Primer name Sequences (5'-3') PeU3-1 F: ATCCGCCCCTGGTTGCTCTA R: CAAGCCGTTAATCACGCTCTGG PeU3-2 F: TCAGCGTTGACCTCCTCTTG R: TGGCACGAAATGGGATGAAG 1301-PeU3-1-1-F F: GACCTGCAGGCATGCAAGCTTTCAGCGTTGACCTCCTCTT R: TTTACCCTCAGATCTACCATTGCTGAGTAGCGCAGGGCTC 1301-PeU3-1-2-F F: GACCTGCAGGCATGCAAGCTCAGGTCATAAGATGGAAGCT R: TTTACCCTCAGATCTACCATTGCTGAGTAGCGCAGGGCTC 1301-PeU3-1-3-F F: GACCTGCAGGCATGCAAGCTCAGCCCATACGAAAGTGATT R: TTTACCCTCAGATCTACCATTGCTGAGTAGCGCAGGGCTC 1301-PeU3-2-1-F F: GACCTGCAGGCATGCAAGCTCCCTAGTGATGCCTTAAAGC R: TTTACCCTCAGATCTACCATTGCATCAATGCTCCAAAGCT 1301-PeU3-2-2-F F: GACCTGCAGGCATGCAAGCTATGGTATTCTGTTGCGGACT R: TTTACCCTCAGATCTACCATTGCATCAATGCTCCAAAGCT 1301-PeU3-2-3-F F: GACCTGCAGGCATGCAAGCTTGGCCTTTTTCATGGAGCCG R: TTTACCCTCAGATCTACCATTGCATCAATGCTCCAAAGCT LUC-PeU3-1-1-F F: TCGACGGTATCGATAAGCTTTTCAGCGTTGACCTCCTCTT R: GCTCTAGAACTAGTGGATCCTGCTGAGTAGCGCAGGGCTC LUC-PeU3-1-2-F F: TCGACGGTATCGATAAGCTTCAGGTCATAAGATGGAAGCT R: GCTCTAGAACTAGTGGATCCTGCTGAGTAGCGCAGGGCTC LUC-PeU3-1-3-F F: TCGACGGTATCGATAAGCTTCAGCCCATACGAAAGTGATT R: GCTCTAGAACTAGTGGATCCTGCTGAGTAGCGCAGGGCTC LUC-PeU3-2-1-F F: TCGACGGTATCGATAAGCTTATCCGCCCCTGGTTGCTCTA R: GCTCTAGAACTAGTGGATCCTGCATCAATGCTCCAAAGCT LUC-PeU3-2-2-F F: TCGACGGTATCGATAAGCTTATGGTATTCTGTTGCGGACT R: GCTCTAGAACTAGTGGATCCTGCATCAATGCTCCAAAGCT LUC-PeU3-2-3-F F: TCGACGGTATCGATAAGCTTTGGCCTTTTTCATGGAGCCG R: GCTCTAGAACTAGTGGATCCTGCATCAATGCTCCAAAGCT