羊草成熟胚诱导愈伤组织及植株再生系统的优化

肖燕,王振兴,李东明,齐艳华,恩和巴雅尔

Optimization of Tissue Culture and Plant Regeneration System of Mature Embryo of Leymus chinensis

Yan Xiao,Zhenxing Wang,Dongming Li,Yanhua Qi, Enhebayaer

表1 不同浓度6-BA和NAA配比下羊草愈伤组织的分化率(平均值±标准误)

Table 1 Differentiation efficiency of Leymus chinensis callus under different concentrations of 6-BA and NAA (means±SE)

6-BA/NAA (mg·L-1) Callus number The number of adventitious buds in proliferation The number of roots in reproduction Differentiation efficiency (%) 0.5/0.25 21 0 0 0 1.0/0.25 21 1A 4+ 4.8±4.8 ab 2.0/0.25 21 0 0 0 3.0/0.25 21 3AB 0 14.3±8.3 abcd 4.0/0.25 21 8AB 0 38.1±4.8 efgh 5.0/0.25 21 4A 0 19.1±4.8 abcde 0.5/0.5 21 0 0 0 1.0/0.5 21 7BC 6+ 33.3±9.5 defg 2.0/0.5 21 10BC 5+ 47.6±9.5 gh 3.0/0.5 21 7AB 4 33.3±4.8 defg 4.0/0.5 21 10AB 0 47.6±9.5 gh 5.0/0.5 21 3BC 0 14.3±0 abcd 0.5/1.0 21 2A 8+ 9.5±4.8 abc 1.0/1.0 21 12AB 4 57.1±4.8 h 2.0/1.0 21 9A 0 42.9±8.3 fgh 3.0/1.0 21 5AC 3 23.8±8.3 bcdef 4.0/1.0 21 4A 1+ 19.1±4.8 abcde 5.0/1.0 21 5A 0 23.8±12.6 bcdef 0.5/2.0 21 5ABC 9+ 23.8±4.8 bcdef 1.0/2.0 21 1A 5 4.8±4.8 ab 2.0/2.0 21 0 0 0 3.0/2.0 21 2A 1+ 9.5±4.8 abc 4.0/2.0 21 2AB 4 9.5±4.8 abc 5.0/2.0 21 0 0 0 0.5/3.0 21 1A 3 4.8±4.8 ab 1.0/3.0 21 4A 3 19.1±4.8 abcde 2.0/3.0 21 7AB 3 33.3±4.8 defg 3.0/3.0 21 10ABC 1 47.6±9.5 gh 4.0/3.0 21 0 0 0 5.0/3.0 21 0 0 0 0.5/4.0 21 6A 3 28.6±8.2 cdefg 1.0/4.0 21 0 0 0 2.0/4.0 21 8AB 2 38.1±4.8 efgh 3.0/4.0 21 0 0 0 4.0/4.0 21 3AB 5 14.3±8.2 abcd 5.0/4.0 21 6AB 0 28.6±8.2 cdefg