图5. 拟南芥RB转基因株系RB-9和RB-12中效应基因Avrblb1致死活性检测
(A) 在拟南芥RB转基因株系RB-9和RB-12中瞬时表达Avrblb1后GUS报告基因表达分析, GFP为对照(红色虚线划分轰击后双管基因枪2个枪孔形成的GUS蓝斑, 黑色数字指示GUS蓝斑数量(Bars=5 mm)); (B) 在拟南芥RB转基因株系中瞬时表达Avrblb1基因后的GUS蓝斑数量统计(拟南芥和本氏烟各10片叶, 实验重复2次, 处理与对照产生GUS斑数进行对数转换后用威尔科克森符号轶和确定统计差异(误差线表示±标准差, P>0.1)); (C) 利用RT-PCR检测拟南芥RB转基因株系RB-9和RB-12中RB基因的表达, UBC9为内参基因。

Figure 5. Measurement of cell death induction by effector gene Avrblb1 in Arabidopsis transgenic line RB-9 and RB-12
(A) Co-bombardment mediated transient expression of Avrblb1 in transgenic lines RB-9 and RB-12, with GFP as control (the red dotted line separates two positions produced by co-bombardment, numbers of GUS spots are indicated in black numbers (Bars=5 mm)); (B) The diagram showed that transient expression of Avrblb1 in RB transgenic lines (ten leaves of each plant were tested, and the test was repeated twice, P values for treatments and the control were calculated from the log ratios using the Wilcoxon rank sum test (error bar represents ± SD, P>0.1)); (C) The RT-PCR detection of RB expressed in transgenic line RB-9 and RB-12, UBC9 was used as the reference gene.