图4. 拟南芥和本氏烟叶片中致病疫霉效应基因Avrblb1和马铃薯抗病基因RB致死活性检测
(A) 拟南芥和本氏烟中双管基因枪介导Avrblb1/Rpp13基因对瞬时表达, GFP为对照, 红色虚线划分轰击后双管基因枪2个枪孔形成的GUS蓝斑, 黑色数字指示GUS蓝斑数量(Bars=5 mm); (B) GUS蓝斑比率(拟南芥和本氏烟各10个叶片, 实验重复2次, 误差线表示±标准差)。处理与对照产生GUS斑数进行对数转换后用威尔科克森符号轶和确定统计差异, ***表示差异极显著(P<0.001); (C) 本氏烟叶片中农杆菌介导Avrblb1/RB瞬时表达, GFP为阴性对照, PAMP分子Inf1为阳性对照, 注射4天后拍照。每个处理重复注射10次(Bar=5 mm)。

Figure 4. Cell death induction triggered by Avrblb1 of Phytophthora infestans and RB of Solanum tuberosum in Arabidopsis thaliana and Nicotiana benthamiana leaves
(A) Co-bombardment mediated transient expression of Avrblb1/Rpp13 gene pair in A. thaliana and N. benthamiana leaves, the red dotted line separates two positions produced by co-bombardment, numbers of GUS spots are indicated in black numbers (Bars=5 mm); (B) Ratio of GUS positive spots (ten leaves from each plant were tested, and the test was repeated twice, error bar represents ± SD). P values for treatments and the control were calculated from the log ratios using the Wilcoxon rank sum test, *** indicates extremely significant differences (P<0.001); (C) Agrobacterium-mediated transient expression of Avrblb1/RB in N. benthamiana leaves, GFP and Inf1 were used as negative and positive control, respectively. Pictures were taken 4 days post infiltration, each treatment contains 10 replicates (Bar=5 mm).