图3. 拟南芥和本氏烟叶片中的病原菌效应基因致死活性检测
=利用双管基因枪在拟南芥和本氏烟叶片上瞬时表达哺乳动物细胞凋亡因子BAX、大豆疫霉菌RxLR效应基因Avh238以及拟南芥霜霉菌效应基因ATR13和拟南芥抗病基因Rpp13后, GUS蓝斑指示的细胞坏死情况, GFP为对照。红色虚线划分轰击后双管基因枪2个枪孔形成的GUS蓝斑, 黑色数字代表形成的GUS蓝斑数量(Bars=5 mm)。

Figure 3. Cell death induction by various pathogen effectors in Arabidopsis thaliana and Nicotiana benthamiana leaves
Mammalian apoptosis inducer BAX, RxLR effector gene Avh238 of Phytophthora sojae, effector gene ATR13 of Hyaloperonospora arabidopsidis and disease resistance gene Rpp13 of A. thaliana gene pairs were transiently expressed in leaves of A. thaliana and N. benthamiana by double-barreled particle bombardment, cell necrosis was indicated by GUS blue spots, with GFP as control. The red dotted line separates two positions produced by co-bombardment, numbers of GUS spots are indicated in black numbers (Bars=5 mm).