双管基因枪介导的基因瞬时表达技术在拟南芥中的应用
赵华1,邵广达1,2,高文鑫1,2,顾彪1,2,*()
The Application of Double-barreled Particle Bombardment for Transient Gene Expression in Arabidopsis
Hua Zhao1,Guangda Shao1,2,Wenxin Gao1,2,Biao Gu1,2,*()

图2. 不同拟南芥植株莲座叶中瞬时表达GUS报告基因的效率
(A) 不同莲座叶中双管基因枪介导GFP/GUS混合报告基因瞬时表达, 红色虚线划分轰击后双管基因枪2个枪孔形成的GUS蓝斑, 黑色数字代表形成的GUS蓝斑数量(Bars=5 mm); (B) 统计分析20片莲座叶中叶尖(Barrel 1)和叶柄(Barrel 2)产生GUS蓝斑的数量(误差线表示±标准差, P>0.1); (C) 将图B中的数据, 按照相同叶片Barrel 1和Barrel 2产生GUS蓝斑数量的比率进行统计分析(误差线表示±标准差)。每次选取2株拟南芥的10个叶片进行测试, 实验重复2次。将Barrel 1与Barrel 2产生GUS蓝斑数量进行对数转换后用威尔科克森符号轶和确定统计差异。

Figure 2. The efficiency of transient expression of GUS gene in different rosette leaves of Arabidopsis thaliana
(A) Co-bombardment of GFP/GUS gene mixture in different rosette leaves, the red dotted line separates two positions produced by double-barreled particle bombardment, numbers of GUS blue spots are indicated in black numbers (Bars=5 mm); (B) Statistic analysis of GUS spots counted from the tip of leaf blade (Barrel 1) and petiole (Barrel 2) (error bar represents ± SD, P>0.1); (C) Ratios of GUS spot numbers in Barrel 1 and Barrel 2 of the same leaf (error bar represents ± SD). Ten rosette leaves from two A. thaliana plants were tested and the test was repeated twice. Statistical analysis by Wilcoxon rank sum test using log ratios of GUS spot numbers in Barrel 1 and Barrel 2.