双管基因枪介导的基因瞬时表达技术在拟南芥中的应用
赵华1,邵广达1,2,高文鑫1,2,顾彪1,2,*(
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The Application of Double-barreled Particle Bombardment for Transient Gene Expression in Arabidopsis
Hua Zhao1,Guangda Shao1,2,Wenxin Gao1,2,Biao Gu1,2,*()
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图1. 利用双管基因枪在拟南芥和本氏烟叶片中瞬时表达GFP和GUS报告基因
(A) 用于基因瞬时表达的拟南芥植株, 红色箭头所示为选用的莲座叶(Bar=5 mm); (B) 拟南芥叶片中GFP报告基因荧光显微观察(Bar=50 μm); (C), (D) 分别为拟南芥和本氏烟叶片中GUS报告基因的表达情况。黑色数字代表统计的GUS蓝斑数量(Bars=5 mm)。
Figure 1. Transient expression of GFP and GUS reporter genes in Arabidopsis thaliana and Nicotiana benthamiana leaves via double-barreled particle bombardment
(A) The rosette leaves (red arrow pointed) of A. thaliana were selected for transient gene expression (Bar=5 mm); (B) Observation of green fluorescent reporter gene (GFP) with fluorescent microscopy (Bar=50 μm); (C), (D) Transient expression of GUS in A. thaliana and N. benthamiana leaves, respectively. Numbers of GUS positive spots are indicated in black numbers (Bars=5 mm).
