双管基因枪介导的基因瞬时表达技术在拟南芥中的应用

赵华,邵广达,高文鑫,顾彪

The Application of Double-barreled Particle Bombardment for Transient Gene Expression in Arabidopsis

Hua Zhao,Guangda Shao,Wenxin Gao,Biao Gu

表1 PCR和qRT-PCR引物序列

Table 1 Primer sequences of PCR and qRT-PCR

Primer name Forward primer (5'-3') Reverse primer (5'-3') GFP CTAGCCCCGGGATGGTGAGCAAGGGCGAG CTGAGGTACCTTACTTGTACAGCTCGTC GUS CTAGCCCCGGGATGGTAGATCTGAGGAACC CTGAGGTACCTCACACGTGATGGTGATGG BAX CTAGCCCCGGGATGGACGGGTCCGGGGAG GTGCAGGATCCCTGGAAGAAGATGGGCTGA Avh238 CTAGCCCCGGGATGGAGGCGAAGCTCTTG GTGCAGGATCCGTTGGCCAAGCCTGTGTAG Avrblb1 CTAGCCCCGGGATGGTTTCATCCAATCTCAAC GTGCAGGTACCCTAGCTAGGGCCAACGTTTTTATC RB CTAGCCCCGGGATGGCTGAAGCTTTCATTCAAG GTGCAGGATCCTTAAATATATATATTCACATTAG ATR13 CTAGCCCCGGGATGAATCTGCTCCACGCCCATG GTGCAGGATCCTTACTGACTGGCAACGGCAGTC Rpp13 CTAGCCCCGGGATGGTAGATGCGATCACGGAG GTGCAGGATCCCTAAACGCTCGCAATCGGTTTG qRB GAGAAGAGCAATTCCCTGTGC GTTAGGTGCTGCAATCCCTCTG qUBC9 CATCGGATAGCCCTTATTCTG TGGAACACCTTCGTCCTAAAA