图1. Col-0野生型拟南芥CCA1pro:LUC在持续红光条件下的生物节律分析
(A) 携带有CCA1pro:LUC报告基因的Col-0野生型拟南芥在持续红光条件下, 从LL24到LL144时间范围内生物发光的荧光节律(c.p.s代表每株植物每秒钟采集到的光子数)。(B) 野生型拟南芥生物钟周期的散点图, X轴表示生物钟周期, Y轴表示相对振幅误差; 根据结果显示每株幼苗周期较为集中, 相对一致, 而且相对振幅误差低于0.5, 结果可靠(一般认为当RAE值大于0.5时, 节律的振荡强度偏低, 不适于计算生物钟周期)。(C) 野生型拟南芥生物钟周期的柱状图; 根据结果显示周期长度在持续红光下为23.22小时。计算方法: FFT-NLLS。数据为21株幼苗的平均值±标准误。

Figure 1. Circadian phenotype analysis of Col-0 wild type Arabidopsis thaliana CCA1pro:LUC under continuous red light
(A) Trace plot of bioluminescence in Col-0 wild type Arabidopsis thaliana with CCA1pro:LUC reporter under continuous red light conditions, from LL24 to LL144 (the abbreviation of c.p.s represents the counts of photons per seedling). (B) Scatter plot of circadian period (x-axis) and relative amplitude error (RAE) (y-axis) of Col-0; According to the results, the periods are more concentrated and relatively consistent, and the relative amplitude errors are all lower than 0.5, which indicates that the result is reliable (generally, when the RAE is greater than 0.5, the circadian oscillation intensity is relatively low, which is not suitable for calculating the period). (C) Estimated circadian period of CCA1pro:LUC in Col-0; According to the calculation, circadian period length of Col-0 is 23.22 hours under continuous red light. Calculation was performed using FFT-NLLS method. Data are presented as means±SE (n=21).