小麦TaLCD基因的克隆及其对渗透胁迫的调节作用
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张扬,刘华杰,薛瑞丽,李海霞,李华
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Cloning of Wheat TaLCD Gene and Its Regulation on Osmotic Stress
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Yang Zhang,Huajie Liu,Ruili Xue,Haixia Li,Hua Li
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表1 qRT-PCR分析所用引物
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Table 1 Primers used for qRT-PCR analysis
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| Gene name | Primer sequences | | Forward (5'-3') | Reverse (5'-3') | | TaDCD | GAGGAAGGACGGAAGCCATAT | TCAGGGTCATCGCAAACAGAG | | TaLCD | TCCATTACGCCTACGGAGCAG | CAAGCCGGACCTTACGACCA | | ABF2 | ATCAGAAGGGATAGGGAAGAGTAAT | TTGGTCTGCCGTGAATATCTGT | | HAB1 | GTGTTCTCGCCATGTCTAGGTC | CTATTTCGCAGACTTCTTGGTTG | | HAB2 | GCAGAAGTCCTTATTGCGAGTC | CTCAGAAGTTGCCACCTCCATA | | ABI1 | TGACGGCTGTGAAGAGAGTA | CCATCTCACACGCTTCTTCA | | ABI2 | ATTCAGACCATTCACTGACCCTC | GCTCCGTCGCCAGAACAAG | | NCED3 | TGGCTTCTTTCACGGCAAC | ACAACAATGGCGGGAGAGTTT | | COR47 | GAGGTTACGGATCGTGGAT | GAGCTGTTGGATCGGTGA | | RAB18 | ATGGCGTCTTACCAGAACCGTCCA | TACCCTTGGCCACCTGATCC | | RD29A | GTTACTGATCCCACCAAAGAAGA | GGAGACTCATCAGTCACTTCCA | | RD22 | AGGGCTGTTTCCACTGAGG | CACCACAGATTTATCGTCAGACA | | Actin2 | TACCCGATGGGCAAGTCA | TGCTCATACGGTCAGCGATA |
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