磷酸肌醇激酶FAB1调控拟南芥根毛伸长
姚玉婷,马家琦,冯晓莉,潘建伟,王超(
)
)
A Role of Arabidopsis Phosphoinositide Kinase, FAB1, in Root Hair Growth
Yuting Yao,Jiaqi Ma,Xiaoli Feng,Jianwei Pan,Chao Wang()
)
图5. 拟南芥fab1b/c/d突变体根毛活性氧(ROS)含量及肌动蛋白稳定性改变
(A) 用DCF-DA (ROS染液)检测Col-0、fab1b/c/d和Col-0- YM201636根毛中的ROS含量(Bar=25 µm); (B) 定量分析DCF-DA荧光强度; (C) Col-0、fab1b/c/d和Col-0-YM201636的根毛肌动蛋白细胞骨架标记ABD2的分布(Bar=25 µm); (D) 定量分析ABD2-GFP荧光强度。*** P<0.001 (Student’s t-test)
Figure 5. Reactive oxygen species (ROS) intensity and actin stability were altered in root hairs of Arabidopsis fab1b/c/d seedlings
(A) Total ROS generated by oxidation of DCF-DA in wild type, fab1b/c/d and Col-0-YM201636 root (Bar=25 µm); (B) Relative intensity of the GFP signals; (C) Distribution of actin cytoskeleton marker ABD2 in root hair of Col-0, fab1b/c/d and Col-0-YM201636 treatment (Bar=25 µm); (D) Average relative intensity of the ABD2-GFP signals. *** P<0.001 (Student’s t-test)
