图1. FAB1B、FAB1C和FAB1D调控拟南芥根毛伸长
(A) FAB1基因结构和T-DNA插入位点; (B) RT-PCR检测FAB1B、FAB1C和FAB1D的表达; (C) 5天龄幼苗根毛长度(黄色虚线框代表定量区域) (Bar=5 mm); (D) 定量根毛长度; (E)-(M) FAB1单突变体、双突变体及YM201636 (FAB1特异性抑制剂)处理的野生型幼苗根毛表型(Bar=0.5 mm); (N) 定量(E)-(M)突变体根毛长度; (O) 根毛形态分析, 红色箭头表示根毛上的凸起和分叉(Bar=75 µm); (P) 定量分叉根毛占总根毛数量的百分比。*** P<0.001 (Student’s t-test)

Figure 1. FAB1B, FAB1C and FAB1D regulate root hair growth in Arabidopsis
(A) FAB1 gene structure and T-DNA insertion sites; (B) Analysis of gene expression of FAB1B, FAB1C and FAB1D by RT-PCR; (C) Assay of root hair length in 5-day-old seedlings (the yellow dotted box represents quantitative area) (Bar=5 mm); (D) Quantification of root hair length; (E)-(M) The root hair phenotype of the FAB1 single mutants, double mutants and YM201636 (FAB1-specific inhibitor) treatment of Col-0 seedling (Bar=0.5 mm); (N) Quantification of (E)-(M) mutant root hair length; (O) Images of the typical root hair morphologies, the root hair morphologies were categorized as swollen or branched (red arrow indicates) (Bar=75 μm); (P) Quantification of frequency of branched root hairs (%). *** P<0.001 (Student’s t-test)