图4. TmAFP转基因甘薯植株的鉴定
(A) 采用除草剂(1.0 mg·L^-1 GAP)对再生植株进行筛选(4-12、4-11、4-10、4-9: 抗性植株; 47-1、47-2: 非抗性植株; CK: 非转基因对照); (B) PCR快速鉴定转基因植株(AFP片段长度353 bp) (M: D2000 DNA marker (下同); +CK: pSUIBEV3-AFP质粒正对照; -CK: 未转化对照植株; 4-9、4-10、4-12: GAP抗性植株; 47-2: 非抗性植株); (C) 转基因植株的PCR检测(+CK: pSUIBEV3-AFP质粒作正对照扩增AFP基因; -CK: 未转化对照植株扩增AFP基因; 4-9-AFP: 转基因植株扩增AFP基因; 4-9-RPB2: 转基因植株扩增RPB2基因; -CK-RPB2: 未转化对照植株扩增RPB2基因); (D) Southern杂交(+CK: pCAMBIA-AFP质粒正对照; -CK: 未转化对照植株; 其余为不同转基因株系); (E) 转基因植株RT-PCR检测(RP1、RP2: 转pCAMBIA空载植株; 4-12、4-11、44-1、54-5: 转pCAMBIA-AFP株系)。

Figure 4. Detection of TmAFP in the transgenic sweet potato plants
(A) Screening with 1.0 mg·L^-1 GAP (Line 4-12, 4-11, 4-10, 4-9: Resistant seedlings; Line 47-1, 47-2: Non-resistant seedlings; CK: Non-transgenic control); (B) Amplified 353-bp fragment of AFP gene (M: D2000 molecular weight marker; +CK: pSUIBEV3-AFP vector as positive control; -CK: Non-transgenic seedlings as negative control; Line 4-9, 4-10, 4-12: GAP resistant seedlings; Line 47-2: Non-resistant seedlings); (C) PCR detection of transgenic seedlings (+CK: Amplifying AFP gene using pSUIBEV3-AFP as template; -CK: Amplifying AFP gene using non-transgenic seedling; 4-9-AFP: Amplifying AFP gene in transgenic seedling; 4-9-RPB2: Amplifying RPB2 gene in transgenic seedling; -CK-RPB2: Amplifying RPB2 gene in non-transgenic seedling); (D) Southern blotting analysis (+CK: pCAMBIA-AFP vector as positive control; -CK: Non-transgenic seedlings as negative control; The others represent different transgenic seedling lines); (E) RT-PCR detection of transgenic seedlings (RP1, RP2: Transgenic seedlings with empty pCAMBIA vector; Line 4-12, 4-11, 44-1, 54-5: Transgenic seedlings with pCAMBIA-AFP vector).