图3. 甘薯抗性体胚及其转基因植株再生过程
(A) 用于转化的甘薯胚性悬浮细胞在MS+0.2 mg·L^-1 2,4-D液体培养基中28°C黑暗振荡培养32周; (B) 选择与非选择培养4周的胚性愈伤组织, a-c为选择性培养基(MS+0.2 mg·L^-1 2,4-D+100 mg·L^-1 Carb+0.8 mg·L^-1 GAP), 箭头所指为抗性愈伤组织; d为未加除草剂的对照; (C) 转基因植株再生过程, a: 再生组织; b: 生芽; c: 生叶; d: 成株; e: 成苗。Bars=1 cm

Figure 3. Sweet potato resistant somatic embryo and the regeneration of transgenic plants
(A) Sweet potato embryogenic suspension cells cultivated in MS+0.2 mg·L^-1 2,4-D liquid medium at 28°C for 32 weeks; (B) Embryogenic callus cultivated in selective and non-selective medium for 4 weeks, a-c: Embryogenic callus cultivated in selective medium (MS+0.2 mg·L^-1 2,4-D+100 mg·L^-1 Carb+0.8 mg·L^-1 GAP), resistant callus was marked by arrows; d: Embryogenic callus cultivated in control medium without herbicide; (C) The processes of transgenic plant regeneration, a: Reproductive tissue; b: Bud; c: Leaf; d: Seedling; e: Reproductive plant. Bars=1 cm