图2. 不同浓度除草剂和抗生素对甘薯胚性愈伤组织和组培苗的影响 (A) 选择培养基上培养8周的甘薯胚性悬浮细胞。从左到右培养基依次为MS+0.2 mg·L-1 2,4-D分别添加0、0.2、0.4、0.6和0.8 mg·L-1 GAP; (B) 培养6周(从左至右第1, 2)和8周(3, 4)的胚性悬浮细胞, 培养基分别为MS+0.2 mg·L-1 2,4-D (1, 3)和MS+0.2 mg·L-1 2,4-D+100 mg·L-1 Carb (2, 4); (C) 培养8周的胚性悬浮细胞, 培养基为MS+0.2 mg·L-1 2,4-D+100 mg·L-1 Carb分别添加0.8、1.0、1.2和1.4 mg·L-1 GAP; (D) 分别为MS+0.2 mg·L-1 2,4-D+0.8 mg·L-1 GAP+100 mg·L-1 Carb培养基上接种0天和培养3周的Huachano茎尖。(A), (B), (C) Bars=1 cm; (D) Bar=2 cm
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Figure 2. Effects of herbicide and antibiotic in different concentrations on embryogenic callus and regenerated seedlings of sweet potato (A) Sweet potato embryogenic suspension cells at 8 weeks cultivated in selective medium. The medium from left to right was MS+0.2 mg·L-1 2,4-D with 0, 0.2, 0.4, 0.6, 0.8 mg·L-1 GAP, respectively; (B) Embryogenic suspension cells at 6 weeks (the first and second from left to right) and 8 weeks (the third and fourth). The medium were MS+0.2 mg·L-1 2,4-D (the first and third) MS+0.2 mg·L-1 2,4-D+100 mg·L-1 Carb (the second and fourth); (C) Embryogenic suspension cells at 8 weeks, the medium are MS+0.2 mg·L-1 2,4-D+100 mg·L-1 Carb with 0.8, 1.0, 1.2, 1.4 mg·L-1 GAP, respectively; (D) Huachano stem tips cultivated 0 day and 3 weeks on medium of MS+0.2 mg·L-1 2,4-D+0.8 mg·L-1 GAP+100 mg·L-1 Carb, respectively. (A), (B), (C) Bars=1 cm; (D) Bar=2 cm
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