转昆虫抗冻蛋白基因增强甘薯抗冻能力
赖先军1,张义正2,古英洪2,颜朗1,*(
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Transformation of Insect Derived Antifreeze Gene into Sweet Potato (Ipomoea batatas) and Enhanced Its Freeze-tolerance
Xianjun Lai1,Yizheng Zhang2,Yinghong Gu2,Lang Yan1,*()
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图1. 甘薯遗传转化体系
(A), (B) 分别在MS+0.2 mg·L-1 2,4-D液体培养基中继代18和22周后的甘薯胚性悬浮细胞; (C) 在MS+0.2 mg·L-1 2,4-D固体培养基上培养8周后的甘薯胚性悬浮细胞; (D) 甘薯体细胞胚再生的小植株。(A), (B), (D) Bars=1 cm; (C) Bar=4 cm
Figure 1. Transformation system of sweet potato
(A), (B) Sweet potato embryogenic suspension cells at 18 and 22 weeks cultivated in MS+0.2 mg·L-1 2,4-D liquid medium, respectively; (C) Sweet potato embryogenic suspension cells at 8 weeks cultivated in MS+0.2 mg·L-1 2,4-D solid medium; (D) Sweet potato seedlings regenerated from somatic embryo. (A), (B), (D) Bars=1 cm; (C) Bar=4 cm
