植物蛋白SUMO化修饰检测方法
曲高平(
),金京波
),金京波
Detection of SUMOylation in Plants
Gaoping Qu(),Jingbo Jin
),Jingbo Jin
图2. 烟草中检测GFP-protein X的SUMO化修饰
GFP-protein X分别与FLAG-SUMO1GG或FLAG-SUMO1AA在烟草叶片中共表达, IP (anti-GFP)产物分别用anti-GFP和anti- FLAG抗体检测。
Figure 2. SUMOylation of GFP-protein X in Nicotiana benthamiana
GFP-protein X was transiently co-expressed with FLAG-SUMO1GG or FLAG-SUMO1AA in Nicotiana benthamiana leaves. GFP-protein X was immunoprecipitated with anti-GFP antibody, and IP products were detected with anti-FLAG antibody.
