植物蛋白SUMO化修饰检测方法
曲高平(
),金京波
),金京波
Detection of SUMOylation in Plants
Gaoping Qu(),Jingbo Jin
),Jingbo Jin
图1. Protein X体外SUMO化修饰
GST-protein X-Myc分别与His-SUMO E1、His-SUMO E2和His- SUMO1GG (His-SUMO1AA作为阴性对照), 进行体外SUMO化反应。反应后用Glutathione beads进一步纯化。Western blot, 分别用anti-Myc和anti-SUMO1抗体进行检测。箭头指示SUMO化修饰的GST-protein X-Myc。
Figure 1. Protein X can be SUMOylated in vitro
GST-protein X-Myc was incubated with His-SUMO E1, His- SUMO E2 and His-SUMO1GG (His-SUMO1AA was used as a negative control) at 30°C for 3 h. After reaction, GST-protein X-Myc was purified with Glutathione beads and detected with anti-Myc and anti-SUMO1 antibodies. Arrows represent SUMOylated GST-protein X-Myc.
