植物蛋白SUMO化修饰检测方法
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曲高平,金京波
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Detection of SUMOylation in Plants
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Gaoping Qu,Jingbo Jin
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表1 SUMO反应相关蛋白的诱导条件
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Table 1 Expression of SUMO reaction related proteins
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| Proteins | IPTG concentration
(mmol·L-1) | Induction time | | His-SUMO E1 | 1 | 16-20 h (16°C) | | His-SUMO E2 | 1 | 3-5 h (28°C) | | His-SUMO1GG | 1 | 16-20 h (16°C) | | His-SUMO1AA | 1 | 16-20 h (16°C) | | GST-protein X-Myc | 1 | 3-5 h (28°C) |
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