图3. 拟南芥幼苗总蛋白双向凝胶电泳后的磷酸化蛋白Pro-Q Diamond染色检测分析
2周龄拟南芥幼苗总蛋白提取液经双向凝胶电泳分离后, 用Pro-Q Diamond试剂对磷酸化蛋白进行染色(左图)。荧光扫描后的凝胶再进行考马斯亮蓝染色(blue-silver staining) (右图)。

Figure 3. Pro-Q staining assay of the phospho-proteins in total proteins extracted from Arabidopsis thaliana seedlings and separated by a 2D gel
Two-week-old Arabidopsis thaliana seedlings were used for total protein extraction. The total proteins were separated by a 2D gel and the phospho-proteins were stained by Pro-Q (left). The gel was scanned with a Typhoon 9410 fluorescence scanner, and then stained with blue-silver to visualize the proteins (right).