图3. 蛋白质OsXLG2-OsBIK1互作实例
将分别含有CLuc-OsXLG2和OsBIK1-NLuc质粒的农杆菌液1:1体积比混合后注射4周龄烟草叶片, 24-48小时后进行检测, 以CLuc-OsPPI和OsBIK1-NLuc作为负对照(未发表)。(A) 利用植物活体成像系统采集烟草叶片的发光图像(Bar=2 cm); (B) 使用luminometer检测萤火素酶活性, 误差表示平均值的标准偏差(n=8); (C) Western blot检测CLuc和NLuc融合蛋白的表达, 融合蛋白分别用anti-luciferase和anti-CLuc抗体来检测。

Figure 3. An example of dynamic OsXLG2-OsBIK1 interactions
Four-week old Nicotiana benthamiana leaves co-infiltrated with Agrobacterium expressing CLuc-OsXLG2 and OsBIK1-NLuc. CLuc-OsPPI and OsBIK1-NLuc are used as negative control (unpublished). (A) The luminescence images were captured using a CCD imaging system (Bar=2 cm); (B) The luciferase activity measurement using a luminometer, the error bars represent the standard deviation from the mean (n=8); (C) Western blot analysis of the expressed proteins of CLuc- and NLuc- fusions, the fusions are detected using anti-luciferase and anti-CLuc antibodies, respectively.