图2. 萤火素酶互补实验示意图
(A) 选取4周龄完全伸展的烟草叶片进行农杆菌注射, 24-48小时后取样观察(Bar=2 cm); (B) 截取完整的农杆菌注射后的烟草叶片, 叶背向上平放于培养皿中(Bar=1 cm); (C) 利用植物活体分子影像系统检测烟草叶片的发光情况并拍照; (D) 选取农杆菌注射后的烟草叶片, 置于培养皿中用打孔器打孔(Bar=1 cm); (E) 将直径为0.6 cm的烟草圆片置于装有200 μL无菌水的96孔酶标板中; (F) 利用luminometer检测烟草叶片的荧光强度。图中圆圈示农杆菌注射部位。

Figure 2. Schematic diagram of the Luciferase Complementation Assay
(A) Select fully expanded young leaves of Nicotiana benthamiana (four-week old) for Agrobacterium injection, observe after 24-48 h (Bar=2 cm); (B) Cut the infiltrated tobacco leaves and put them into the petri dish (Bar=1 cm); (C) Use CCD imaging system to detect the luminescence of the complete tobacco leaves and captured the pictures; (D) Select infiltrated leaves for punch (Bar=1 cm); (E) Punch tobacco leaf discs of 0.6 cm in diameter from infiltrated leaf areas by a cork borer and place leaf discs in a 96-well plate containing 200 μL sterile water in each well; (F) Use luminometer to scan the fluorescence intensity of the tobacco leaves. The circles show the injection sites of Agrobacterium.