图1. 表达载体示意图
(A) pCAMBIA1300-NLuc (pNL); (B) pCAMBIA1300-CLuc (pCL); (C) pCAMBIA1300-HA-NLuc (pHNL); (D) pCAMBIA1300-CCLuc (pCCL)。构建的表达载体中均包含pCAMBIA1300骨架, NLuc位于目标基因C末端, CLuc位于目标基因的N末端或C末端; 序列中显示的是单酶切位点。MCS: 多克隆酶切位点; L: 连接序列; RBS: 转录终止子; ATG: 翻译起始密码子; TGA: 翻译终止密码子; HA: 标签蛋白

Figure 1. Plasmid maps
(A) pCAMBIA1300-NLuc (pNL); (B) pCAMBIA1300-CLuc (pCL); (C) pCAMBIA1300-HA-NLuc (pHNL); (D) pCAMBIA1300-CCLuc (pCCL). All the vectors have a framework of pCAMBIA1300 and allow expression of the target genes with a C-terminal NLuc fusion and N-terminal or C-terminal CLuc fusion. Unique sites are shown in sequence. MCS: Multiple cloning sites; L: Linker sequence; RBS: Transcription terminator; ATG: Translation initiation codon; TGA: Translation stop codon; HA: Tag protein