植物中验证蛋白相互作用的Pull-down和Co-IP技术
徐重益(
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Pull-down and Co-immunoprecipitation Assays of Interacting Proteins in Plants
Chongyi Xu()
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图1. 利用原核表达系统检测融合GST ( 谷胱甘肽-S-转移酶)的诱饵蛋白与捕获蛋白互作的Pull-down实验流程图
IPTG: 异丙基硫代-β-D-半乳糖苷; GSH: 谷胱甘肽
Figure 1. Schematic representation of the Pull-down procedure in prokaryotic expression system to test the interaction between a bait protein tagged with GST (glutathione S-transferase) and a prey protein
IPTG: Isopropyl-β-D-thiogalactopyranoside; GSH: Glutathione
