甘薯盐胁迫响应基因IbMYB3的表达特征及生物信息学分析

李格,孟小庆,李宗芸,朱明库

Expression Patterns and Bioinformatic Analyses of Salt Stress Responsive Gene IbMYB3 in Ipomoea batatas

Ge Li,Xiaoqing Meng,Zongyun Li,Mingku Zhu

表1 实验所用引物

Table 1 Primers used in the experiment

Primer name Primer sequence (5'-3') FIbMYB3-F ATGGGAAGATCTCCATGCTG FIbMYB3-R TTTACAGCAAATCTTCGAAATCTA FIbMYB4-F ATGGGGAGATCACCATGCT FIbMYB4-R TTGTTCTGGACAAATATCTACAGAA QIbMYB3-F GCCAGCCAACTTGAGTACCG QIbMYB3-R AAGAACCGAGTCCAACCCG QIbMYB4-F ATCCACTCCCACCTTGTACGAC QIbMYB4-R TCCAAAACAGCCGCCATAGT QIbARF-F CTTTGCCAAGAAGGAGATGC QIbARF-R TCTTGTCCTGACCACCAACA IbMYB3-EGFP- XbaI-F CCGTCTAGAATGGGAAGATCTCCATGCTGT IbMYB3-EGFP- BamHI-R CGCGGATCCCAGCAAATCTTCGAAATCTAAG IbMYB3-BD-F CCGGAATTCATGGGAAGATCTCCATGCTGT IbMYB3-BD-R CGCGGATCCTTACAGCAAATCTTCGAAATCTAAG