图位克隆技术新型遗传学实验教学项目的设计与实践

陈析丰,刘亚萍,马伯军

Design and Practice of a New Teaching Project of the Map-based Cloning Experiment in Genetics

Xifeng Chen,Yaping Liu,Bojun Ma

表1 本研究SSR标记的PCR引物

Table 1 PCR primers of the SSR markers used in this study

SSR标记 正向引物 (5°-3°) 反向引物 (5°-3°) RM21103 ACAAGTCCACAAGGACCACAACC TGCTCCACCCAAAGATACAGAGC RM21309 GAGTGGATTTACTGACAAGCTCTTCC GATTCTCTTGGAGCCCATACTCC RM21470 TCTTGCCATCACATAGCAACAGG ACTCGGTGAGCATCCAATGTCC RM22426 ATTTCGTCGTCGTGTCTGTCC TTAAAGCAGCCTGATCGAAAGC RM22648 AGCAGCTCCGATTGTGTTAGCC AATGGCGTCTGTGGTACTTTGC RM22925 CGATTACCAGGACAACAGATCACG GTGGCTCTTGAGTGTACGTGTGC