金黄花滇百合植株再生与离体快繁技术体系的建立
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张文婷,何燕红,舒宁,邢景景,刘宝骏,包满珠,刘国锋
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Plant Regeneration and Rapid Propagation System of Lilium bakerianum var. aureum
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Wenting Zhang,Yanhong He,Ning Shu,Jingjing Xing,Baojun Liu,Manzhu Bao,Guofeng Liu
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表1 金黄花滇百合不同部位外植体再生能力比较
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Table 1 Comparison of regeneration capabilities of different explants from Lilium bakerianum var. aureum
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| Treatments | Explants | 6-BA
(mg∙L-1) | NAA
(mg∙L-1) | No. of
explants | Survival
rate (%) | No. of induced buds | No. of induced callus | | 1 | Bulbs | 0 | 0 | 8 | 100 | 0 | 0 | | 2 | Bulbs | 0.5 | 0.5 | 15 | 80.0 | 3 | 11 | | 3 | Scale | 0 | 0 | 30 | 10.0 | 3 | 0 | | 4 | Scale | 0.5 | 0.1 | 10 | 30.0 | 3 | 0 | | 5 | Scale | 0.5 | 0.5 | 30 | 0 | 0 | 0 | | 6 | Scale | 2.0 | 0.5 | 30 | 23.3 | 2 | 7 | | 7 | Scale | 1.5 | 0.5 | 10 | 20.0 | 2 | 0 | | 8 | Callus | 0 | 0 | 13 | 23.1 | 2 | 13 (browning) | | 9 | Callus | 0.5 | 0.1 | 30 | 83.3 | 53 | 30 (swelled obviously) | | 10 | Callus | 0.5 | 0.5 | 24 | 62.5 | 46 | 24 (callus proliferation) | | 11 | Callus | 2.0 | 0.5 | 15 | 80.0 | 14 | 15 (bulbs differentiation) | | 12 | Callus | 1.0 | 0.1 | 22 | 81.8 | 71 | 22 (budding obviously) | | 13 | Petiole | 0.5 | 0.1 | 118 | 5.9 | 0 | 7 | | 14 | Petiole | 0.5 | 0.5 | 60 | 6.7 | 1 | 3 | | 15 | Petiole | 2.0 | 0.5 | 60 | 0 | 0 | 0 | | 16 | Petiole | 1.0 | 0.1 | 90 | 4.4 | 9 | 0 | | 17 | Leaf | 0.5 | 0.1 | 60 | 0 | 0 | 0 | | 18 | Leaf | 0.5 | 0.5 | 60 | 0 | 0 | 0 | | 19 | Leaf | 2.0 | 0.5 | 60 | 0 | 0 | 0 |
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