植物蛋白的体外泛素化检测方法
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赵庆臻 1,刘利静 2,谢旗 3,4,于菲菲 3,*( )
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In Vitro Ubiquitination Assay for Plant Proteins
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Qingzhen Zhao 1,Lijing Liu 2,Qi Xie 3,4,Feifei Yu 3,*( )
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图4. SDIR1-SDIRIP1的E3-底物泛素化反应(Zhang et al., 2015) MBP-SDIR1和GST-MYC-SDIRIP1共同孵育进行体外泛素化反应。反应产物用anti-MYC进行western blot检测。
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Figure 4. E3-substrate ubiquitination assay of SDIR1-SDIRIP1 pair (Zhang et al., 2015) MBP-SDIR1 and GST-MYC-SDIRIP1 was incubated together for in vitro ubiquitination assay. The western blot was analyzed using anti-MYC antibody.
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