植物蛋白的体外泛素化检测方法
赵庆臻1,刘利静2,谢旗3,4,于菲菲3,*()
In Vitro Ubiquitination Assay for Plant Proteins
Qingzhen Zhao1,Lijing Liu2,Qi Xie3,4,Feifei Yu3,*()

图4. SDIR1-SDIRIP1的E3-底物泛素化反应(Zhang et al., 2015)
MBP-SDIR1和GST-MYC-SDIRIP1共同孵育进行体外泛素化反应。反应产物用anti-MYC进行western blot检测。

Figure 4. E3-substrate ubiquitination assay of SDIR1-SDIRIP1 pair (Zhang et al., 2015)
MBP-SDIR1 and GST-MYC-SDIRIP1 was incubated together for in vitro ubiquitination assay. The western blot was analyzed using anti-MYC antibody.