植物蛋白的体外泛素化检测方法
赵庆臻1,刘利静2,谢旗3,4,于菲菲3,*(
)
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In Vitro Ubiquitination Assay for Plant Proteins
Qingzhen Zhao1,Lijing Liu2,Qi Xie3,4,Feifei Yu3,*()
)
图3. 不同E2-E3特异性相互作用(
将一种E3分别与不同E2组合(A, B), 或者将同一种E2分别与不同的E3组合(C, D)进行体外泛素化反应。其中, (A)和(B)中UBCH5b为阳性对照, (C)和(D)中-E3及MBP为阴性对照。反应产物用anti-His抗体进行western blot检测。泳道下方的+代表多聚泛素链的生成。
Figure 3. The specificity of various E2-E3 combinations (
The ubiquitination activity was detected at the case of one E3 combined with different E2s (A, B) or one E2 combined with different E3s (C, D). UBCH5b is positive control in (A) and (B) while -E3 and MBP are negative control in (C) and (D). The outcome of reaction was detected by western blot with anti-His antibody. Plus symbols below the lanes indicate the formation of poly-Ub chains.
