快速高效检测植物体内蛋白泛素化修饰研究方法
刘利静1,赵庆臻2,谢旗3,4,于菲菲3,*()
An Quick and Efficient Assay for In Vivo Protein Ubiquitination
Lijing Liu1,Qingzhen Zhao2,Qi Xie3,4,Feifei Yu3,*()

图7. 烟草注射法表达的蛋白可用于体外泛素化实验(Liu et al., 2010)
(A) 利用anti-GFP检测样品的体外泛素化情况; (B) 利用Nickel-HRP检测样品的体外泛素化情况。★为COP1自身泛素化及HY5泛素化条带的混合物。▲为泛素化的E2。左侧数字是蛋白marker的分子量(kDa)。IgG H: IgG重链蛋白

Figure 7. Proteins expressed in tobacco can be used for in vitro ubiquitination reaction (Liu et al., 2010)
(A) Immunoblots of the in vitro ubiquitination assays samples with anti-GFP antibody; (B) Immunoblots of the in vitro ubiquitination assays samples with Nickel-HRP to detect His-ubiquitin. ★ The mixture of poly-ubiquitinated HY5-GFP and Myc-COP1. ▲ Mono-ubiquitinated E2. The numbers on the left show the molecular masses of marker proteins in kiloDaltons (kDa). IgG H: IgG heavy chain