快速高效检测植物体内蛋白泛素化修饰研究方法
刘利静1,赵庆臻2,谢旗3,4,于菲菲3,*(
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An Quick and Efficient Assay for In Vivo Protein Ubiquitination
Lijing Liu1,Qingzhen Zhao2,Qi Xie3,4,Feifei Yu3,*()
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图2. 烟草注射法检测底物蛋白HY5和ELF3体内的自身泛素化情况(
(A) 利用anti-MYC抗体对免疫沉淀的样品进行western blot检测; (B) 利用anti-Ub抗体对免疫沉淀的样品进行western blot检测。左侧数字是蛋白marker的分子量(kDa)。
Figure 2. Polyubiquitinated MYC-HY5 and MYC-ELF3 can be detected in Nicotiana benthamiana (
(A) Immunoblots of immunoprecipitated samples with anti- MYC antibody; (B) Immunoblots of immunoprecipitated samples with anti-Ub antibody. The numbers on the left show the molecular masses of marker proteins in kiloDaltons (kDa).
