快速高效检测植物体内蛋白泛素化修饰研究方法
刘利静1,赵庆臻2,谢旗3,4,于菲菲3,*(
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An Quick and Efficient Assay for In Vivo Protein Ubiquitination
Lijing Liu1,Qingzhen Zhao2,Qi Xie3,4,Feifei Yu3,*()
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图1. 用烟草注射法检测HY5与COP1之间的相互作用(
(A) 利用anti-MYC抗体进行免疫共沉淀反应, 用anti-GFP抗体进行western blot检测; (B) 利用anti-GFP抗体进行免疫共沉淀反应, 用anti-MYC抗体进行western blot检测。左侧数字是蛋白marker的分子量(kDa); IgG H: IgG重链蛋白
Figure 1. HY5 and COP1 interact with each other in Nicotiana benthamiana (
(A) Samples were immunoprecipitated with anti-MYC antibody and immunoblotted with anti-GFP antibody; (B) Samples were immunoprecipitated with anti-GFP antibody and immunoblotted with anti-MYC antibody. The numbers on the left show the molecular masses of marker proteins in kiloDaltons (kDa). IgG H: IgG heavy chains
