水稻两性生殖细胞的N-甲基-N-亚硝基脲诱变方法
田怀东,李菁,田保华,牛鹏飞,李珍,岳忠孝,屈雅娟,姜建芳,王广元,岑慧慧,李南,闫枫

Method for N-methyl-N-nitrosourea Mutagenesis on Hermaphroditic Germ Cells of Rice
Huaidong Tian,Jing Li,Baohua Tian,Pengfei Niu,Zhen Li,Zhongxiao Yue,Yajuan Qu,Jianfang Jiang,Guangyuan Wang,Huihui Cen,Nan Li,Feng Yan
表3 辽盐6号水稻生殖细胞的MNU处理因素对M1-M2群体中突变性状表达的影响
Table 3 Effects of MNU-treatment factors on mutant-character expression in M1-M2 populations of germ cells of Liaoyan 6 rice cultivar
Treatments of
germ cells		 Number of mutant characters Incidences (%)
Seedling Culm Leaf Panicle Glume Seed Total
Variable
MNU
(mmol∙L-1)		 0.5
1.0
1.5
2.0		 3
4
8
5		 2
4
6
3		 3
4
5
2		 1
2
3
1		 1
1
2
-		 8
15
15
6		 18
30
39
17		 4.6
7.6
9.9
4.3
Variable
time
(min)		 20
40
60
80		 3
4
5
5		 2
4
3
3		 3
4
5
3		 2
2
1
2		 -
1
2
1		 5
15
15
6		 15
30
31
20		 3.8
7.6
7.9
5.1
Variable
pH		 4.2
4.8
5.4
6.0		 5
4
5
3		 3
4
3
3		 2
4
3
3		 1
2
1
-		 1
1
1
1		 9
15
10
6		 21
30
23
16		 5.3
7.6
5.9
4.1
Variable
PO43-
(mmol∙L-1)		 5
10
20
40		 5
4
6
5		 2
4
3
3		 3
4
4
2		 2
2
1
1		 1
1
2
1		 7
15
7
6		 20
30
23
18		 5.1
7.6
5.9
4.6
Control treatment 3 2 2 1 1 4 13 3.3