水稻两性生殖细胞的N-甲基-N-亚硝基脲诱变方法
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田怀东,李菁,田保华,牛鹏飞,李珍,岳忠孝,屈雅娟,姜建芳,王广元,岑慧慧,李南,闫枫
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Method for N-methyl-N-nitrosourea Mutagenesis on Hermaphroditic Germ Cells of Rice
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Huaidong Tian,Jing Li,Baohua Tian,Pengfei Niu,Zhen Li,Zhongxiao Yue,Yajuan Qu,Jianfang Jiang,Guangyuan Wang,Huihui Cen,Nan Li,Feng Yan
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表2 MNU处理因素对辽盐6号水稻生殖细胞M1群体生长发育的影响
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Table 2 Effects of MNU-treatment factors on growth and development of M1 populations of germ cells of Liaoyan 6 rice cultivar
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Treatments
of germ cells | Setting
rate (%) | Seedling
rate (%) | Adult plant rate (%) | Fertility
rate(%) | Variable
MNU
(mmol∙L-1) | 0.5
1.0
1.5
2.0 | 86.1
78.6
73.5
62.2 | 82.3
74.5
68.3
59.8 | 82.4
77.1
70.4
61.7 | 89.3
80.4
76.7
68.2 | Variable
time
(min) | 20
40
60
80 | 88.5
78.6
74.7
65.8 | 83.1
74.5
69.3
60.8 | 83.2
77.1
72.3
66.9 | 91.0
80.4
73.8
67.6 | Variable
pH | 4.2
4.8
5.4
6.0 | 85.3
78.6
74.5
82.9 | 80.3
74.5
79.6
83.7 | 85.3
77.1
81.0
84.9 | 89.6
80.4
85.2
87.9 | Variable
PO43-
(mmol∙L-1) | 5
10
20
40 | 83.7
78.6
82.5
87.4 | 82.4
74.5
78.1
85.0 | 86.3
77.1
70.6
82.8 | 89.7
80.4
85.3
86.9 | | Control treatment | 89.5 | 87.3 | 90.4 | 92.8 |
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