水稻两性生殖细胞的N-甲基-N-亚硝基脲诱变方法

田怀东,李菁,田保华,牛鹏飞,李珍,岳忠孝,屈雅娟,姜建芳,王广元,岑慧慧,李南,闫枫

Method for N-methyl-N-nitrosourea Mutagenesis on Hermaphroditic Germ Cells of Rice

Huaidong Tian,Jing Li,Baohua Tian,Pengfei Niu,Zhen Li,Zhongxiao Yue,Yajuan Qu,Jianfang Jiang,Guangyuan Wang,Huihui Cen,Nan Li,Feng Yan

表1 水稻生殖细胞MNU实验设计

Table 1 Design for MNU treatments of germ cells in rice

Treatments MNU (mmol∙L-1) Time (min) pH PO43– (mmol∙L-1) Germ cells 0.5 1.0 1.5 2.0 20 40 60 80 4.2 4.8 5.4 6.0 5 10 20 40 Control 1.0 40 4.8 10