水稻早衰突变体LS-es1的基因定位及候选基因分析

周纯,焦然,胡萍,林晗,胡娟,徐娜,吴先美,饶玉春,王跃星

Gene Mapping and Candidate Gene Analysis of Rice Early Senescence Mutant LS-es1

Chun Zhou,Ran Jiao,Ping Hu,Han Lin,Juan Hu,Na Xu,Xianmei Wu,Yuchun Rao,Yuexing Wang

表1 基因定位及qRT-PCR所用引物序列

Table 1 Primers used for gene mapping and qRT-PCR

Primer name Forward primer (5'-3') Reverse primer (5'-3') InDel-1 AGCGGGGATGGAGATGATG CTTGCCTCACACCAGATCTG InDel-2 GGCGCCTTTGTTCCATAGTT GAGGAGCCAGTGGTAGCAG InDel-3 CGTTTTTACAACCAATTTTGGAA CCATCTTCTACCTCCGGACA InDel-4 GATTGGATTGGTTGCTCGCT AACAGCGAATCGAGATGCAC InDel-5 TTACTGCTGCCGTTGTTTCA TTGTGGACCTCCAGGATCAG SGR AGGGGTGGTACAACAAGCTG GCTCCTTGCGGAAGATGTAG Osh36 GCACGGAGGCGAACGA TTGAGCGGTAGCACCCATT Osl85 GAGCAACGGCGTGGAGA GCGGCGGTAGAGGAGATG OsNAP CAAGAAGCCGAACGGTTC GTTAGAGTGGAGCAGCAT Actin CAGGCCGTCCTCTCTCTGTA AAGGATAGCATGGGGGAGAG